Enrichment of CD56dimKIR+CD57+ highly cytotoxic NK cells in tumor infiltrated lymph nodes of melanoma patients
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چکیده
from the TILN suspension of one individual patient within the cohort to demonstrate gating strategy. First, on the left the major lymphocyte cell gate was defined by excluding tumour cells, granulated cells, dead cells, and debris by size (FSC) and granularity (SSC). Next, in the second plot CD3 and CD56 double staining was used to exclude T (CD3+) and NKT (CD56+CD3+) cells, and to sequentially identify NK cells as CD3-CD56+ population. Finally in the third plot on the right, either bright (CD56bright, upper region) or dim (CD56dim, lower region) for CD56 staining can be distinguished.
منابع مشابه
Enrichment of CD56(dim)KIR + CD57 + highly cytotoxic NK cells in tumour-infiltrated lymph nodes of melanoma patients.
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